Molecular and functional characterization of macrophage migration inhibitory factor (MIF) homolog of human from lymphatic filarial parasite Wuchereria bancrofti.
Identifieur interne : 004108 ( Main/Exploration ); précédent : 004107; suivant : 004109Molecular and functional characterization of macrophage migration inhibitory factor (MIF) homolog of human from lymphatic filarial parasite Wuchereria bancrofti.
Auteurs : Rohit Sharma [Inde] ; S L Hoti ; R L Meena ; V. Vasuki ; T. Sankari ; P. KalirajSource :
- Parasitology research [ 1432-1955 ] ; 2012.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antihelminthe (sang), Cadres ouverts de lecture, Clonage moléculaire, Escherichia coli (génétique), Exons, Expression des gènes, Facteurs inhibiteurs de la migration des macrophages (génétique), Facteurs inhibiteurs de la migration des macrophages (métabolisme), Filariose lymphatique (immunologie), Filariose lymphatique (parasitologie), Humains, Immunoglobuline E (sang), Introns, Protéines d'helminthes (génétique), Protéines d'helminthes (métabolisme), Protéines recombinantes (génétique), Protéines recombinantes (isolement et purification), Protéines recombinantes (métabolisme), Wuchereria bancrofti (génétique), Wuchereria bancrofti (isolement et purification).
- MESH :
- génétique : Escherichia coli, Facteurs inhibiteurs de la migration des macrophages, Protéines d'helminthes, Protéines recombinantes, Wuchereria bancrofti.
- immunologie : Filariose lymphatique.
- isolement et purification : Protéines recombinantes, Wuchereria bancrofti.
- métabolisme : Facteurs inhibiteurs de la migration des macrophages, Protéines d'helminthes, Protéines recombinantes.
- parasitologie : Filariose lymphatique.
- sang : Anticorps antihelminthe, Immunoglobuline E.
- Animaux, Cadres ouverts de lecture, Clonage moléculaire, Exons, Expression des gènes, Humains, Introns.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (blood), Cloning, Molecular, Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (parasitology), Escherichia coli (genetics), Exons, Gene Expression, Helminth Proteins (genetics), Helminth Proteins (metabolism), Humans, Immunoglobulin E (blood), Introns, Macrophage Migration-Inhibitory Factors (genetics), Macrophage Migration-Inhibitory Factors (metabolism), Open Reading Frames, Recombinant Proteins (genetics), Recombinant Proteins (isolation & purification), Recombinant Proteins (metabolism), Wuchereria bancrofti (genetics), Wuchereria bancrofti (isolation & purification).
- MESH :
- chemical , blood : Antibodies, Helminth, Immunoglobulin E.
- genetics : Escherichia coli, Helminth Proteins, Macrophage Migration-Inhibitory Factors, Recombinant Proteins, Wuchereria bancrofti.
- immunology : Elephantiasis, Filarial.
- chemical , isolation & purification : Recombinant Proteins, Wuchereria bancrofti.
- chemical , metabolism : Helminth Proteins, Macrophage Migration-Inhibitory Factors, Recombinant Proteins.
- parasitology : Elephantiasis, Filarial.
- Animals, Cloning, Molecular, Exons, Gene Expression, Humans, Introns, Open Reading Frames.
Abstract
The ability of nematode parasites to survive in a highly complex immune system involves diverse strategies including production of a variety of host immune modulators. Various parasite-associated surface antigens or excretory and secretory products may possibly play a role in the host-parasite interactions and successful survival of parasite in their respective host. One among these molecules is a human cytokine homolog, macrophage migration inhibitory factor-1 (MIF-1) in various parasites. We identified a homolog of this cytokine from human lymphatic filarial parasite, Wuchereria bancrofti, expression cloned and investigated its molecular characteristics and catalytic properties. We also assessed the humoral reactivity of the recombinant MIF-1 of W. bancrofti (rWb-MIF-1) against sera belonging to different categories of individuals viz. microfilaremic, chronic patients, endemic normal, and non-endemic normal. Our results showed that the complete coding sequence of W. bancrofti is 1,078 bp, comprising two introns and three exons: first and second introns being 577 and 153 bp long, while the three exons I, II, and III being 108, 173, and 67 bp long, respectively. The rWb-MIF-1 was overexpressed in a salt-inducible host, Escherichia coli GJ 1158, and its functional activity was determined by dopachrome tautomerase and insulin reduction assays. The results of both the assays showed that the purified protein is functionally active and hence folded appropriately. The rWb-MIF-1 protein did not show elevation of specific IgG4 antibodies in microfilaremic cases, a hallmark in case of lymphatic filariasis, while it showed IgE reactivity in some of these cases (five out of ten).
DOI: 10.1007/s00436-012-3051-2
PubMed: 22875393
Affiliations:
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Le document en format XML
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Kaliraj</name></author></analytic><series><title level="j">Parasitology research</title><idno type="eISSN">1432-1955</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (blood)</term><term>Cloning, Molecular</term><term>Elephantiasis, Filarial (immunology)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Escherichia coli (genetics)</term><term>Exons</term><term>Gene Expression</term><term>Helminth Proteins (genetics)</term><term>Helminth Proteins (metabolism)</term><term>Humans</term><term>Immunoglobulin E (blood)</term><term>Introns</term><term>Macrophage Migration-Inhibitory Factors (genetics)</term><term>Macrophage Migration-Inhibitory Factors (metabolism)</term><term>Open Reading Frames</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (isolation & purification)</term><term>Recombinant Proteins (metabolism)</term><term>Wuchereria bancrofti (genetics)</term><term>Wuchereria bancrofti (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antihelminthe (sang)</term><term>Cadres ouverts de lecture</term><term>Clonage moléculaire</term><term>Escherichia coli (génétique)</term><term>Exons</term><term>Expression des gènes</term><term>Facteurs inhibiteurs de la migration des macrophages (génétique)</term><term>Facteurs inhibiteurs de la migration des macrophages (métabolisme)</term><term>Filariose lymphatique (immunologie)</term><term>Filariose lymphatique (parasitologie)</term><term>Humains</term><term>Immunoglobuline E (sang)</term><term>Introns</term><term>Protéines d'helminthes (génétique)</term><term>Protéines d'helminthes (métabolisme)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (isolement et purification)</term><term>Protéines recombinantes (métabolisme)</term><term>Wuchereria bancrofti (génétique)</term><term>Wuchereria bancrofti (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term><term>Immunoglobulin E</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>Helminth Proteins</term><term>Macrophage Migration-Inhibitory Factors</term><term>Recombinant Proteins</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term><term>Facteurs inhibiteurs de la migration des macrophages</term><term>Protéines d'helminthes</term><term>Protéines recombinantes</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Recombinant Proteins</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines recombinantes</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Helminth Proteins</term><term>Macrophage Migration-Inhibitory Factors</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Facteurs inhibiteurs de la migration des macrophages</term><term>Protéines d'helminthes</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Immunoglobuline E</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cloning, Molecular</term><term>Exons</term><term>Gene Expression</term><term>Humans</term><term>Introns</term><term>Open Reading Frames</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cadres ouverts de lecture</term><term>Clonage moléculaire</term><term>Exons</term><term>Expression des gènes</term><term>Humains</term><term>Introns</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The ability of nematode parasites to survive in a highly complex immune system involves diverse strategies including production of a variety of host immune modulators. Various parasite-associated surface antigens or excretory and secretory products may possibly play a role in the host-parasite interactions and successful survival of parasite in their respective host. One among these molecules is a human cytokine homolog, macrophage migration inhibitory factor-1 (MIF-1) in various parasites. We identified a homolog of this cytokine from human lymphatic filarial parasite, Wuchereria bancrofti, expression cloned and investigated its molecular characteristics and catalytic properties. We also assessed the humoral reactivity of the recombinant MIF-1 of W. bancrofti (rWb-MIF-1) against sera belonging to different categories of individuals viz. microfilaremic, chronic patients, endemic normal, and non-endemic normal. Our results showed that the complete coding sequence of W. bancrofti is 1,078 bp, comprising two introns and three exons: first and second introns being 577 and 153 bp long, while the three exons I, II, and III being 108, 173, and 67 bp long, respectively. The rWb-MIF-1 was overexpressed in a salt-inducible host, Escherichia coli GJ 1158, and its functional activity was determined by dopachrome tautomerase and insulin reduction assays. The results of both the assays showed that the purified protein is functionally active and hence folded appropriately. The rWb-MIF-1 protein did not show elevation of specific IgG4 antibodies in microfilaremic cases, a hallmark in case of lymphatic filariasis, while it showed IgE reactivity in some of these cases (five out of ten).</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Hoti, S L" sort="Hoti, S L" uniqKey="Hoti S" first="S L" last="Hoti">S L Hoti</name><name sortKey="Kaliraj, P" sort="Kaliraj, P" uniqKey="Kaliraj P" first="P" last="Kaliraj">P. Kaliraj</name><name sortKey="Meena, R L" sort="Meena, R L" uniqKey="Meena R" first="R L" last="Meena">R L Meena</name><name sortKey="Sankari, T" sort="Sankari, T" uniqKey="Sankari T" first="T" last="Sankari">T. Sankari</name><name sortKey="Vasuki, V" sort="Vasuki, V" uniqKey="Vasuki V" first="V" last="Vasuki">V. Vasuki</name></noCountry><country name="Inde"><noRegion><name sortKey="Sharma, Rohit" sort="Sharma, Rohit" uniqKey="Sharma R" first="Rohit" last="Sharma">Rohit Sharma</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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